AMBMP is a small molecule that has been previously reported to be both a Wnt agonist and a microtubule (MT) regulator. We showed that a detailed analysis of AMBMPs effects on MTs and on MT associated cellular processes including cell polarity, ciliogenesis, and cell migration. Specifically, treatment of Xenopus embryos with AMBMP leads to defects similar to the MT depolymerizing drug nocodazole, including a failure to generate or polarize
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MT-associated proteins (MAPs) have context-dependent roles in regulating their stability and dynamics. The poorly characterized clamp/Spef1 gene encodes a MAP and is expressed in several tissues and cell types. We have shown that CLAMP dynamically interacts with the microtubule latice and promotes promotes centriole positioning and radial intercalation of multiciliated cells by connecting microtubule organization to apico-basal polarity.
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Mamalian cylicin is localized to the cytoskeletal calyx that surrounds part of the nucleus in the head of sperm. The two highly conserved proteins, CYLC-1 and CYLC-2 in C.elegans have been suggested to function in the intracellular compartmentalization of tubulin levels in uterine muscle cells. We generated CRISPR tagged worm strains for both genes and show that both proteins are highly enriched in sperm in both hermaphrodites and males concentrated in puncta presumed to be membranous organelles.
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